Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: Counts per million (CPM) for the indicated transcripts from the RNAseq analysis in . ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Duplicate RNA samples from independent crosses were submitted for each genotype to the Plasmidsaurus RNAseq analysis pipeline.

Techniques: RNA sequencing

Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: A) Table summarizing the overall count of differentially regulated genes in RNAseq analyses performed in isolated fat bodies where the indicated RNAi lines are driven by Dcg-GAL4 . B) Venn-diagram showing the commonalities in the differentially downregulated (left) and upregulated genes (right) in A. C-E) Counts per million (CPM) for the indicated transcripts from the RNAseq analysis in A . C and D show data for known EcR targets, E shows data for known ATF4 targets. Also see Table S1 and S2 . ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Duplicate RNA samples from independent crosses were submitted for each genotype to the Plasmidsaurus RNAseq analysis pipeline.

Techniques: RNA sequencing, Isolation

Journal: The Journal of Clinical Investigation

Article Title: The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect

doi: 10.1172/JCI170369

Figure Lengend Snippet: ( A – B ) Lineage tracing of chondrocytes coexpressing mutant Ifitm5 and Ai9 reporter in Rosa 26 mIfitm5/Ai9 Acan-Cre ERT2 mice. Tamoxifen injection was performed at P10, and bones were collected after 3 weeks (at 5 weeks of age). Representative images of the proximal tibia ( A ) and the tibia diaphyseal area ( B ) Decreased fraction of Ai9 + cells migrated to the bone diaphysis in mutant animals compared with littermate controls ( n = 3 per genotype; n = 2–3 fields counted per sample; * P = 0.03, by 2-tailed Student’s t test). Original magnification, ×20. ( C ) RNA-Seq of total femoral cDNA from Rosa26 mIfitm5/+ Acan-Cre ERT2 mice ( n = 3 per genotype) showed enrichment for chondrogenic gene expression in the mutant samples by GO term analysis. ( D ) Heatmap of focused differential gene expression analysis from RNA-Seq, showing upregulation of chondrogenic gene markers and decreased expression of osteogenic markers in the mutants compared with the control group.

Article Snippet: Differential gene expression analysis was performed using DESeq2, and statistical significance was determined by 2-way ANOVA, which is built into the Partek Genomics Suite RNA-Seq analysis pipeline.

Techniques: Mutagenesis, Injection, RNA Sequencing Assay, Expressing, Control